Nathalie GABORIT - TRICHEREAU

Through the analysis of transcriptional regulation, my research program aims to (1) decipher the mechanisms by which physiological cardiac electrical activity is established and maintained, and thus create a solid basis for (2) the study of the mechanisms of cardiac arrhythmias.

RESEARCH EXPERIENCES

• From 2023 : Research Director - l'institut du thorax, Nantes, France - CNRS Commission #24
• 2021 : Awarded HDR (Habilitation à Diriger des Recherches) - Nantes University, France
• 2015 - 2023 : Primary investigator - l'institut du thorax, Nantes, France - CNRS Commission #24
• 2012 - 2014: Post-doctoral fellow - l'institut du thorax, Nantes, France
• 2006 - 2011: Post-doctoral fellow - Gladstone Institute of Cardiovascular Disease, San Francisco, USA
• 2006: Awarded PhD in biology from Université de Nantes, France
• 2002 - 2006: PhD student - l'institut du thorax, Nantes, France

 

 RESEARCH PUBLICATIONS

• Author of 28 articles in peer-reviewed journals (average IF = 6.4)
• Hirsch (H) index: 16 (data from Web of Science)
• Average citations per article : 69 (max. 568, Google Scholar)
• ResearchGate profile
• Google Scholar profile
• ORCID profile : 0000-0002-5231-6555

Articles from 2015

1. Canac R*, Cimarosti B*, Girardeau A, Forest V, Olchesqui P, Poschmann J, Redon R, Lemarchand P, Gaborit N*, Lamirault G*. Deciphering transcriptional networks during human cardiac development. Cells. 2022;11:3915.
2. Caillaud A, Levêque A, Thédrez A, Girardeau A, Canac R, Bray L, Baudic M, Rimbert A, Barc J, Gaborit N, Lamirault G, Gardie B, Le May C, Cariou B, Si-Tayeb K. FACS-assisted CRISPR-Cas9 genome editing of human induced pluripotent stem cells. Star Protocols. 2022;3:101680.
3. Canac R, Caillaud A, Cimarosti B, Girardeau A, Hamamy H, Reversade B, Bonnard C, Al Sayed ZR, David L, Poschmann J, Lemarchand P, Lamirault G*, Gaborit N*. Generation of three human induced pluripotent stem cell lines with IRX5 knockout and knockin genetic editions using CRISPR-Cas9 system. Stem Cell Research. 2022;58:102627.
4. Cimarosti B*, Canac R*, De Waard S, Girardeau A, Gaignerie A, Derevier A, Forest V, De Waard M, Le Marec H, Gourraud J-B, Lemarchand P, Lamirault G*, Gaborit N*. Generation of human induced pluripotent stem cell lines from three patients affected by Catecholaminergic Polymorphic Ventricular Tachycardia (CPVT) carrying heterozygous mutations in RYR2 gene. Stem Cell Research. 2022;60:102688.
5. Cimarosti B, Canac R, Girardeau A, Arnaud M, Francheteau Q, Vincent P, Lemarchand P, Redon R, Gourraud J-B, Gaborit N*, Lamirault G*. Generation of human induced pluripotent stem cell lines from two patients affected by catecholamine-induced QT prolongation (CIQTP). Stem Cell Research. 2021;59:102649.
6. Girardeau A*, Atticus D*, Canac R, Cimarosti B, Caillaud A, Chariau C, Simonet F, Cariou B, Charpentier F, Gourraud J-B, Probst V, Belbachir N, Jesel L, Lemarchand P, Barc J, Redon R, Gaborit N*, Lamirault G*. Generation of human induced pluripotent stem cell lines from four unrelated healthy control donors carrying European genetic background. Stem Cell Research. 2021;59:102647.
7. Al Sayed ZR, Jouni M, Gourraud JB, Belbachir N, Barc J, Girardeau A, Forest V, Derevier A, Gaignerie A, Chariau C, Cimarosti B, Canac R, Olchesqui P, Charpentier E, Schott JJ, Redon R, Baró I, Probst V, Charpentier F, Loussouarn G, Zibara K, Lamirault G, Lemarchand P, Gaborit N. A consistent arrhythmogenic trait in Brugada syndrome cellular phenotype. Clin and Trans Med. 2021;11:e413.
8. Al Sayed ZR, Canac R, Cimarosti B, Bonnard C, Gourraud JB, Hamamy H, Kayserili H, Girardeau A, Jouni M, Jacob N, Gaignerie A, Chariau C, David L, Forest V, Marionneau C, Charpentier F, Loussouarn G, Lamirault G, Reversade B, Zibara K, Lemarchand P, Gaborit N. Human model of IRX5 mutations reveals key role for this transcription factor in ventricular conduction. Cardiovasc Res. 2021;117:2092-2107.
9. De Waard S, Montnach J, Cortinovis C, Chkir O, Erfanian M, Hulin P, Gaborit N, Lemarchand P, Mesirca P, Bidaud I, Mangoni M, De Waard M. Maurocalcin analogue MCaE12A facilitates Ca2+ mobilization in cardiomyocytes. Biochem J. 2020;477:3985-3999.
10. Oualid A, Al Sayed ZR, Stéphane S, Christophe M, Faivre JF, Gaborit N, Chatelier A, Bois P. In vitro differentiation of W8B2+ human cardiac stem cells: ion channels gene expression and spontaneous calcium activity. Cell Mol Biol Letters. 2020;25:50.
11. De Waard S, Montnach J, Ribeiro B, Nicolas S, Forest V, Charpentier F, Mangoni M, Gaborit N, Ronjat M, Loussouarn G, Lemarchand P, De Waard M. Functional impact of BeKm-1, a high affinity hERG blocker, on cardiomyocytes derived from human-induced pluripotent stem cells. Int J Mol Sciences. 2020;21:7167.
12. Belbachir N, Portero V, Al Sayed ZR, Gourraud JB, Dilasser F, Jesel L, Guo H, Wu H, Gaborit N, Guilluy C, Girardeau A, Bonnaud S, Simonet F, Karakachoff M, Pattier S, Scott C, Burel S, Marionneau C, Chariau C, Gaignerie A, David L, Genin E, Deleuze JF, Dina C, Sauzeau V, Loirand G, Baró I, Schott JJ, Probst V, Wu JC, Redon R, Charpentier F, Le Scouarnec S. RRAD mutation causes electrical and cytoskeletal defects in cardiomyocytes derived from a familial case of Brugada syndrome. Eur Heart J. 2019;40:3081-3094.
13. Gaignerie A, Lefort N, Rousselle M, Forest-Choquet V, Flippe L, Francois-Campion V, Girardeau A, Caillaud A, Chariau C, Francheteau Q, Derevier A, Chaubron F, Knöbel S, Gaborit N, Si-Tayeb K, David L. Urine-derived cells provide a readily accessible cell type for feeder-free mRNA reprogramming. Sci Rep. 2018;8:14363.
14. Etienne P, Huchet F, Gaborit N, Barc J, Thollet A, Kyndt F, Guyomarch B, Le Marec H, Charpentier F, Schott JJ, Redon R, Probst V, Gourraud JB. Mental stress test: a rapid, simple, and efficient test to unmask long QT syndrome. Europace. 2018;20:2014-2020.
15. Rima M, Daghsni M, De Waard S, Gaborit N, Fajloun Z, Ronjat M, Mori Y, Brusés JL, De Waard M. The β(4) subunit of the voltage-gated calcium channel (Cacnb4) regulates the rate of cell proliferation in Chinese Hamster Ovary cells. Int J Biochem Cell Biol. 2017;89:57-70.
16. Es-Salah-Lamoureux Z*, Jouni M*, Malak OA, Belbachir N, Al Sayed ZR, Gandon-Renard M, Lamirault G, Gauthier C, Baró I, Charpentier F, Zibara K, Lemarchand P, Beaumelle B, Gaborit N*, Loussouarn G*. HIV-Tat induces a decrease in I(Kr) and I(Ks)via reduction in phosphatidylinositol-(4,5)-bisphosphate availability. J Mol Cell Cardiol. 2016;99:1-13.
17. Cain CJ, Gaborit N, Lwin W, Barruet E, Ho S, Bonnard C, Hamamy H, Shboul M, Reversade B, Kayserili H, Bruneau BG, Hsiao EC. Loss of Iroquois homeobox transcription factors 3 and 5 in osteoblasts disrupts cranial mineralization. Bone Rep. 2016;5:86-95.
18. Jouni M, Si-Tayeb K, Es-Salah-Lamoureux Z, Latypova X, Champon B, Caillaud A, Rungoat A, Charpentier F, Loussouarn G, Baró I, Zibara K, Lemarchand P, Gaborit N. Toward Personalized Medicine: Using Cardiomyocytes Differentiated from Urine-Derived Pluripotent Stem Cells to Recapitulate Electrophysiological Characteristics of Type 2 Long QT Syndrome. J Am Heart Assoc. 2015;4:e002159.

RESEARCH SKILLS

•  Cell culture: culture of cos7 et 10T1/2 cells; cellular transfection; culture of human iPScells; differentiation of human iPS cells in osteoblast, cardiomyocyte andendocardial cells; culture of mouse embryonic stem cells; differentiation ofmouse stem cells into cardiomyocytes; optimization of the iPS cell-osteoblastdifferentiation protocol.
•  Cell Biology: immunohitochemistry;FACS; cell sorting; fluorescence microscopy; confocal miscroscopy.
•  Molecular biology: cloning; DNA microarray; Real-time PCR and TaqMan array; western blot; luciferase assay; chromatinimmunoprecipitation; co-immunoprecipitation; manipulation of BacterialArtificial Chromosome. 
•  Histology: mouse embryo and adult heart dissection; paraffin and OCT embedding of mouseembryonic and adult tissues; paraffin sectioning; cryosectioning;immunofluorescence; in situ hybridization on tissue section; Eosin-Hematoxylineand Acetylcholinesterase section staining.
•  Animal handling: management of mouse colony; mouse ultrasound andembryo dating; production of transgenic mouse lines.
•  Electrophysiology: 6-lead electrocardiogram and telemetry recording and analysis; surgicalimplantation of telemetry device in mouse.

RESEARCH FUNDINGS

• 2022-2026 : ANR PRC
• 2021-2023 : Fédération Française de Cardiologie
• 2019-2023 : Inserm cross‐cutting program
• 2018-2019 : HUGO - Fondation Maladies Rares
• 2015-2019 : ANR Jeune Chercheuse
• 2015-2016 : Fédération Française de Cardiologie
• 2013-2015: Marie Curie IIF
• 2012-2013 : Fédération Française de Cardiologie
• 2013: Fondation Genavie
• 2012-2013 : Fondation Lefoulon-Delalande
• 2010-2012 : American Heart Association
• 2010-2013 : Tobacco-Related Disease Research Program (declined)
• 2006-2007 : Foundation for Medical Research
• 2003-2006 : Région Pays de le Loire

DISTINCTIONS

• 2022: CNRS Bronze medal, Nantes, France
• 2011: Best poster price - CVRI retreat, Santa Cruz, USA
• 2010: Leadership award -Gladstone Institute of Cardiovascular Disease, San Francisco, USA
• 2009: Above and Beyondaward - Gladstone Institute of Cardiovascular Disease, San Francisco, USA
• 2006: Best poster price - GRRC, Toulouse, France